Acetyl-CoA acyltransferase, peroxisomal (ACAA1) monoclonal antibody
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Catalog No. MS716
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$325.00 - 100 µg
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This antibody can also be purchased as part of a Sample Pack.
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Product Specifications
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Applications:
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Western blotting, Immunocytochemistry (heat-induced antigen-retrieval improves signal), Immunoprecipitation, In-Cell ELISA
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Species Reactivity:
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human, rat, mouse
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Host Species:
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mouse
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Isotype:
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IgG1, κ
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Clone ID:
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2H6BE7
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Concentration:
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1 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
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Suggested Working Concentration:
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1 µg/mL for Western blotting
5 µg/mL for Immunocytochemistry
1 µg/mL for In-Cell ELISA (0.1 µg/well)
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Storage Conditions:
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Store at 4°C. Do not freeze.
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Country of Origin:
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USA
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WB Images
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Figure 1. As a Western blotting antibody, MS716 antibody works with human, rat, and mouse samples.
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ICC Images
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Figure 2. Immunocytochemistry image of MS716 stained human HepG2 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were pretreated with heated antigen retrieval buffer (100 mM Tris, 5% urea, PH 9.5, 95°C, 10 min). The cells were incubated with the antibody (2H6BE7, 5 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in peroxisome.
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