ATP synthase subunit alpha monoclonal antibody
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Catalog No. MS507
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$325.00 - 100 µg
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This antibody can also be purchased as part of a Sample Pack.
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UniProt Number:
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P25705
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Alternate Names:
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Complex V, F1F0 ATPase
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Structure and Function:
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ATP synthase is responsible for ATP production in oxidative phosphorylation and can work in reverse as a proton pumping ATPase. The enzyme was thought to be localized exclusively to mitochondria. However, it has recently been identified also on the plasma membrane of several cell types including hepatocytes where it is the HDL receptor, and on endothelial cells where it may act as the angiostatin receptor. The enzyme in mammals is composed of 17 subunits, 5 of which make up the easily detached F1. The remainder are components of 2 stalk domains and the proton pumping F0 part of the machinery. Two of the subunits of the F0 part are encoded on mtDNA while the other subunits are nuclear encoded.
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Product Specifications
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Applications:
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Western blotting, Immunocytochemistry
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Species Reactivity:
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human, bovine, mouse, monkey, rat, Drosophila, C. elegans, oyster
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Host Species:
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mouse
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Isotype:
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IgG2b, κ
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Clone ID:
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15H4C4
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Concentration:
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1 mg/mL in Hepes -Buffered Saline (HBS) with 0.02% azide as a preservative.
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Suggested Working Concentration:
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1 µg/mL for Western blotting
1-2 µg/mL for Immunocytochemistry
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Storage Conditions:
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Store at 4°C. Do not freeze.
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Country of Origin:
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USA
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WB Images
(click to enlarge)
Figure 1. Isolated mitochondria from human heart (lane 1), bovine heart (lane 2), rat heart (lane 3), mouse heart (lane 4), and HepG2 (lane 5) detected with (MS507) anti-subunit alpha antibody.
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ICC Images
(click to enlarge)
Figure 2. Mitochondrial localization of ATP synthase visualized by immunocytochemistry using anti-ATP synthase subunit alpha mAb 15H4C4 (MS507). Cultured human fibroblasts were fixed, permeabilized and then labeled with MS507 followed by a fluorescent goat-anti-mouse IgG.
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Published Studies Using This Product:
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Nadanaciva et al., 2010. High-Content Screening for Compounds That Affect mtDNA-Encoded Protein Levels in Eukaryotic Cells.
Brys et al., 2010. Disruption of insulin signalling preserves bioenergetic competence of mitochondria in ageing Caenorhabditis elegans.
Khanna et al., 2010. Towards a membrane proteome in Drosophila: a method for the isolation of plasma membrane.
Ziviani et al., 2010. Drosophila parkin requires PINK1 for mitochondrial translocation and ubiquitinates mitofusin.
Liu et al., 2009. Functional analysis of FSP27 protein regions for lipid droplet localization, caspase-dependent apoptosis, and dimerization with CIDEA.
Cox et al., 2009. Clueless, a conserved Drosophila gene required for mitochondrial subcellular localization, interacts genetically with parkin.
Yang et al., 2009. Insulin stimulates Akt translocation to mitochondria: implications on dysregulation of mitochondrial oxidative phosphorylation in diabetic myocardium.
Greiss et al., 2008. C. elegans SIR-2.1 translocation is linked to a proapoptotic pathway parallel to cep-1/p53 during DNA damage-induced apoptosis.
Khidr et al.,2008. Role of SUV3 helicase in maintaining mitochondrial homeostasis in human cells.
Mast et al., 2008. Reactive oxygen species act remotely to cause synapse loss in a Drosophila model of developmental mitochondrial encephalopathy.
Yang et al., 2008. C-reactive protein augments hypoxia-induced apoptosis through mitochondrion-dependent pathway in cardiac myocytes.
Kim et al., 2008. Increased expression of the F(1)F(o) ATP synthase in response to iron in heart mitochondria.
Watanabe et al., 2008. Adherent monomer-misfolded SOD1.
Rea et al., 2007. Relationship between mitochondrial electron transport chain dysfunction, development, and life extension in Caenorhabditis elegans.
Li et al., 2007. An assembled complex IV maintains the stability and activity of complex I in mammalian mitochondria.
Park et al., 2007. Yeast NDI1 improves oxidative phosphorylation capacity and increases protection against oxidative stress and cell death in cells carrying a Leber's hereditary optic neuropathy mutation.
Ventura et al., 2007. Caenorhabditis elegans mitochondrial mutants as an investigative tool to study human neurodegenerative diseases associated with mitochondrial dysfunction.
Cortés-Hernández et al., 2007. ATP6 homoplasmic mutations inhibit and destabilize the human F1F0-ATP synthase without preventing enzyme assembly and oligomerization.
Stiburek et al., 2007. Knockdown of human Oxa1l impairs the biogenesis of F1Fo-ATP synthase and NADH:ubiquinone oxidoreductase.
Wall et al., 2006. Alterations in oxidative phosphorylation complex proteins in the hearts of transgenic mice that overexpress the p38 MAP kinase activator, MAP kinase kinase 6.
McQuibban et al., 2006. Normal mitochondrial dynamics requires rhomboid-7 and affects Drosophila lifespan and neuronal function.
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