Catalase monoclonal antibody
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Catalog No. MS721
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$325.00 - 100 µg
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This antibody can also be purchased as part of a Sample Pack.
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Product Specifications
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Applications:
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Immunocytochemistry, Immunoprecipitation, In-Cell ELISA
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Species Reactivity:
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human, bovine, rat
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Host Species:
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mouse
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Isotype:
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IgG1, κ
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Clone ID:
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12C2DB9
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Concentration:
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1.0 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
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Suggested Working Concentration:
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5 µg/mL for Immunocytochemistry
4 µg/mL for In-Cell ELISA (0.4 µg/well)
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Storage Conditions:
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Store at 4°C. Do not freeze.
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Country of Origin:
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USA
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ICC Images
(click to enlarge)
Figure 1. Immunocytochemistry image of MS721 stained human HepG2 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (12C2DB9, 5µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in peroxisome.
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IP Images
(click to enlarge)
Figure 2. Catalase immunocaptured from HepG2 cells, bovine heart sample, and rat liver sample using MS721 anti-catalase monoclonal antibody.
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