Complex I subunit NDUFB4 monoclonal antibody
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Catalog No. MS107
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$325.00 - 100 µg
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This antibody can also be purchased as part of a Sample Pack.
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UniProt Number:
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O95168
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Alternate Names:
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NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4, NADH-ubiquinone oxidoreductase B15 subunit, Complex I-B15 (Short name=CI-B15)
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Structure and Function:
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There are 45 unique subunits of Complex I, and this subunit is 129 AA long. Previously, based on MW and isoelectric point on gels MS107 was tentatively identified as likely reacting against subunit NDUFA6; mass spectrometry now confirms identification of it as subunit NDUFB4. It is thought that this subunit does not contribute to catalytic functions of Complex I.
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Product Specifications
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Applications:
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Western blotting, Immunocytochemistry (heat-induced antigen-retrieval improves signal)
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Species Reactivity:
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human, bovine, rat (weak)
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Host Species:
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mouse
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Isotype:
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IgG1, κ
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Clone ID:
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17G3D9E12
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Concentration:
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1 mg/mL in Hepes-Buffered Saline (HBS)-Buffered Saline (HBS) with 0.02% azide as a preservative.
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Suggested Working Concentration:
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0.5 µg/mL for Western blotting
5-15 µg/mL for Immunocytochemistry
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Storage Conditions:
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Store at 4°C. Do not freeze.
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Country of Origin:
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USA
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WB Images
(click to enlarge)
Figure 1. Isolated mitochondria from human heart (lane 1), bovine heart (lane 2), and rat heart (lane 3), detected with (MS107) anti-NDUFB4 antibody.
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ICC Images
(click to enlarge)
Figure 2. Mitochondrial localization of complex I visualized by immunocytochemistry using anti-complex I subunit NDUFB4 mAb 17G3D9E12 (MS107). Cultured human fibroblasts were fixed, permeabilized and then labeled with MS107 followed by a fluorescent goat-anti-mouse IgG.
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Published Studies Using This Product:
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Thomas et al., 2010. Recombinant human mitochondrial transcription factor A stimulates mitochondrial biogenesis and ATP synthesis, improves motor function after MPTP, reduces oxidative stress and increases survival after endotoxin.
Arthur et al., 2009. Parkinson's disease brain mitochondria have impaired respirasome assembly, age-related increases in distribution of oxidative damage to mtDNA and no differences in heteroplasmic mtDNA mutation abundance.
Keeney et al., 2009. Mitochondrial gene therapy augments mitochondrial physiology in a Parkinson's disease cell model.
Iyer et al., 2009. Recombinant mitochondrial transcription factor A with N-terminal mitochondrial transduction domain increases respiration and mitochondrial gene expression.
Hakonen et al., 2008. Infantile-onset spinocerebellar ataxia and mitochondrial recessive ataxia syndrome are associated with neuronal complex I defect and mtDNA depletion.
Schilling et al., 2005. Rapid purification and mass spectrometric characterization of mitochondrial NADH dehydrogenase (Complex I) from rodent brain and a dopaminergic neuronal cell line.
Murray et al., 2003. The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification.
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