Products > Antibody Cocktails > MitoProfile® Total OXPHOS Human WB Antibody Cocktail

MitoProfile® Total OXPHOS Human WB Antibody Cocktail

Catalog No. MS601

$595.00 - 360 µg
$945.00 - 720 µg


Product Description

Contains 5 mAbs, one each against Complex I subunit NDUFB8 (MS105), Complex II subunit 30kDa (MS203), Complex III subunit Core 2 (MS304), Complex IV subunit II (MS405), and ATP synthase subunit alpha (MS507) as an optimized premixed cocktail. The cocktail is suitable for Western Blotting analysis of the relative levels of the 5 OXPHOS complexes in mitochondrial preparations from human sources. Human heart mitochondria are also included as a Western Blotting control.

Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress.

The mAbs in the cocktail were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used (see protocols).



Specifications
 
Images
 
Documentation
 
Citations

Product Specifications
  
Targets: • Complex I subunit NDUFB8 - "CI-20" ~ 20kD
• Complex II-FeS subunit 30kDa - "CII-30" ~ 30kD
• Complex III subunit Core 2 - "CIII-core2" ~ 47kD
• Complex IV subunit II - "CIV-II" ~ 24kD
• ATP synthase subunit alpha - "CV-alpha" ~ 53kD
Species Reactivity: human
Storage Conditions: 4°C
Country of Origin: USA


Top



Specifications
 
Images
 
Documentation
 
Citations
WB Images



(click to enlarge)

Figure 1. Each lane contains 10 µg of mitochondria isolated from MRC5 fibroblasts. MS601 was used to label lane 6, and the individual antibodies comprising the cocktail were used for lanes 1-5.


Top



Specifications
 
Images
 
Documentation
 
Citations

Downloadable Documents


   Technical Data Sheet

   Protocol


Top



Specifications
 
Images
 
Documentation
 
Citations

Published Studies Using This Product: Thomas et al., 2010. Recombinant human mitochondrial transcription factor A stimulates mitochondrial biogenesis and ATP synthesis, improves motor function after MPTP, reduces oxidative stress and increases survival after endotoxin.

Carilla-Latorre et al., 2010. MidA is a putative methyltransferase that is required for mitochondrial complex I function.

Pennington et al., 2010. Differential effects of wild-type and A53T mutant isoform of alpha-synuclein on the mitochondrial proteome of differentiated SH-SY5Y cells.

Cho et al., 2010. Suppression of ROS generation by 4,4-diaminodiphenylsulfone in non-phagocytic human diploid fibroblasts.

van der Westhuizen et al., 2010. Aberrant synthesis of ATP synthase resulting from a novel deletion in mitochondrial DNA in an African patient with progressive external ophthalmoplegia.

Guillet et al., 2010. Adenine nucleotide translocase is involved in a mitochondrial coupling defect in MFN2-related Charcot-Marie-Tooth type 2A disease.

Beck Jorgensen et al., 2009. Reduced AMP-activated protein kinase activity in mouse skeletal muscle does not exacerbate the development of insulin resistance with obesity.

Raharijaona et al., 2009. PGC-1-related coactivator modulates mitochondrial-nuclear crosstalk through endogenous nitric oxide in a cellular model of oncocytic thyroid tumours.

Zhang et al., 2009. Peripheral neuropeptide Y Y1 receptors regulate lipid oxidation and fat accretion.

Costford et al., 2009. Skeletal Muscle NAMPT is Induced by Exercise in Humans.

Favier et al., 2009. The Warburg effect is genetically determined in inherited pheochromocytomas.

Aguilera-Aguirre et al., 2009. Mitochondrial dysfunction increases allergic airway inflammation.

Baughman et al., 2009. A computational screen for regulators of oxidative phosphorylation implicates SLIRP in mitochondrial RNA homeostasis.

Arthur et al., 2009. Parkinson's disease brain mitochondria have impaired respirasome assembly, age-related increases in distribution of oxidative damage to mtDNA and no differences in heteroplasmic mtDNA mutation abundance.

Fragaki et al., 2009. A neonatal polyvisceral failure linked to a de novo homoplasmic mutation in the mitochondrially encoded cytochrome b gene.

Onopiuk et al., 2009. Mutation in dystrophin-encoding gene affects energy metabolism in mouse myoblasts.

Chu et al., 2009. Regulation of Bax by c-Jun NH2-terminal kinase and Bcl-xL in vinblastine-induced apoptosis.

Keeney et al., 2009. Mitochondrial gene therapy augments mitochondrial physiology in a Parkinson's disease cell model.

Su et al., 2009. Mitochondrial dysfunction in CA1 hippocampal neurons of the UBE3A deficient mouse model for Angelman syndrome.

Oswald et al., 2009. Knockdown of human COX17 affects assembly and supramolecular organization of cytochrome c oxidase.

Iyer et al., 2009. Recombinant mitochondrial transcription factor A with N-terminal mitochondrial transduction domain increases respiration and mitochondrial gene expression.

Nouette-Gaulain et al., 2009. Erythropoietin protects against local anesthetic myotoxicity during continuous regional analgesia.

Vercauterenet al., 2009. Short hairpin RNA-mediated silencing of PRC (PGC-1-related coactivator) results in a severe respiratory chain deficiency associated with the proliferation of aberrant mitochondria.

Upreti et al., 2008. Identification of the major phosphorylation site in Bcl-xL induced by microtubule inhibitors and analysis of its functional significance.

Leroy et al., 2008. Protective effect of eicosapentaenoic acid on palmitate-induced apoptosis in neonatal cardiomyocytes.

Khidr et al., 2008. Role of SUV3 helicase in maintaining mitochondrial homeostasis in human cells.

Samudio et al., 2008. The warburg effect in leukemia-stroma cocultures is mediated by mitochondrial uncoupling associated with uncoupling protein 2 activation.

Huffman et al., 2008. Effect of exercise and calorie restriction on biomarkers of aging in mice.

Soane et al., 2008. Postnatal developmental regulation of Bcl-2 family proteins in brain mitochondria.

van Raam et al., 2008. Mitochondrial membrane potential in human neutrophils is maintained by complex III activity in the absence of supercomplex organisation.

Rodenburg et al., 2008. Impaired barrier function by dietary fructo-oligosaccharides (FOS) in rats is accompanied by increased colonic mitochondrial gene expression.

Liang et al., 2008. Alzheimer's disease is associated with reduced expression of energy metabolism genes in posterior cingulate neurons.

de Wilde et al., 2008. Short-term high fat-feeding results in morphological and metabolic adaptations in the skeletal muscle of C57BL/6J mice.

Barceló-Batllori et al., 2008. Integration of DIGE and bioinformatics analyses reveals a role of the antiobesity agent tungstate in redox and energy homeostasis pathways in brown adipose tissue.

Prahl et al., 2008. Aging skin is functionally anaerobic: importance of coenzyme Q10 for anti aging skin care.

Dlasková et al., 2008. Oxidative stress caused by blocking of mitochondrial complex I H(+) pumping as a link in aging/disease vicious cycle.

Turner et al., 2007. Excess lipid availability increases mitochondrial fatty acid oxidative capacity in muscle: evidence against a role for reduced fatty acid oxidation in lipid-induced insulin resistance in rodents.

Xie et al., 2007. The mitochondrial inner membrane protein mitofilin exists as a complex with SAM50, metaxins 1 and 2, coiled-coil-helix coiled-coil-helix domain-containing protein 3 and 6 and DnaJC11.

Folbergrová et al., 2007. Mitochondrial complex I inhibition in cerebral cortex of immature rats following homocysteic acid-induced seizures.

Heilbronn et al., 2007. Markers of mitochondrial biogenesis and metabolism are lower in overweight and obese insulin-resistant subjects.

Molero et al., 2006. Genetic ablation of the c-Cbl ubiquitin ligase domain results in increased energy expenditure and improved insulin action.

Valla et al., 2006. Impaired platelet mitochondrial activity in Alzheimer's disease and mild cognitive impairment.

Wall et al., 2006. Alterations in oxidative phosphorylation complex proteins in the hearts of transgenic mice that overexpress the p38 MAP kinase activator, MAP kinase kinase 6.


Top



Browse Products By:
Search:


Companion Products:
• Complex I subunit NDUFB8
  monoclonal antibody (cat.#MS105)

• Complex II subunit 30 kDa Ip
  monoclonal antibody (cat.#MS203)

• Complex III subunit Core 2
  monoclonal antibody (cat.#MS304)

• Complex IV subunit II
  monoclonal antibody (cat.#MS405)

• ATP synthase subunit alpha
  monoclonal antibody (cat.#MS507)



Sales & Customer Support:
1-800-910-6486
sales@mitosciences.com

Copyright © 2010 MitoSciences Inc. All rights reserved.